Jpn. J. Infect. Dis., 59 (5), 323-325, 2006
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Marburgvirus Nucleoprotein-Capture Enzyme-Linked Immunosorbent Assay Using Monoclonal Antibodies to Recombinant Nucleoprotein: Detection of Authentic Marburgvirus
Masayuki Saijo*, Marie-Claude Georges-Courbot1, Shuetsu Fukushi, Tetsuya Mizutani, Marianneau Philippe1, Alain-Jean Georges2, Ichiro Kurane and Shigeru Morikawa
Department of Virology 1, National Institute of Infectious Diseases, Tokyo 208-0011, Japan; 1Unit of Biology of Viral Emerging Infections, Insitute Pasteur; and 2Laboratory P4 Jean-Merieux-Inserm, Lyon, France
(Received April 26, 2006. Accepted June 19, 2006)
*Corresponding author: Mailing address: Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan. Tel: +81-42-561-0071 ext. 320, Fax: +81-42-561-2039, E-mail: email@example.com
SUMMARY: There have recently been large outbreaks of Marburg hemorrhagic fever (MHF) caused by Marburgvirus (MARV) in the Democratic Republic of Congo and Angola. The development of reliable diagnostic systems for MHF is urgently needed. An antigen-capture enzyme-linked immunosorbent assay (Ag-capture ELISA) using either of the two monoclonal antibodies (2A7 and 2H6) produced by immunizing mice with recombinant nucleoprotein of MARV was described (Journal of Medical Virology, 76, 111-118, 2005). In the present study, it was revealed that the Ag-capture ELISA specifically detected authentic MARV antigen and that the sensitivity of the Ag-capture ELISA was at a level similar to that of reverse-transcription polymerase chain reaction. These results suggest that the Ag-capture ELISA using the monoclonal antibodies, 2A7 and 2H6, is applicable to the diagnosis of MHF.
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