Jpn. J. Infect. Dis., 59 (5), 341-345, 2006
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Short Communication
Detection and Characterizatin of Variant Salmonella Genomic Island 1s from Salmonella Derby Isolates
Masato Akiba*, Kotaro Nakamura1, David Shinoda2, Noriyo Yoshii, Hiroya Ito3, Ikuo Uchida4 and Muneo Nakazawa5*
Sagfety Research Team and 5Epidemiological Research Team, National Institute of Animal Health, Ibaraka 305-0856; 1Kurayoshi Livestock Hygiene Service Center, Tottori 682-0017; 2Gifu Livestock Hygiene Service Center, Gifu 500-8388; 3Kyushu Research Station, National Institute of Animal Health, Kagoshima 891-0105; and 4Hokkaido Research Station, National Institute of Animal Health, Sapporo 062-0045, Japan
(Received April 28, 2006. Accepted August 4, 2006)
*Corresponding author: Mailing address: Safety Research Team, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, ibaraki 305-0856, Japan. Fax: +81-29-838-7825, E-mail: akiba@affrc.go.jp
SUMMARY: The purpose of this study is to investigate the distribution and structure of Salmonella genomic island 1 (SGI1) among Salmonella enterica serovar Derby isolates from swine and their rearing environment. Three variants of SGI1s, specifically SGI1-A, C, and I, were identified by PCR mapping. The results of macro-restriction analysis and DNA sequencing of SGI1 flanking regions revealed that there are at least two genomic lineages of Derby strains bearing SGI1s.