Jpn. J. Infect. Dis., 60 (1), 45-47, 2007
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Construction of a Pair of Practical Nocardia-Escherichia coli Shuttle Vectors
Kazuhiro Chiba, Yasutaka Hoshino, Keiko Ishino, Takahisa Kogure1, Yuzuru Mikami1, Yoshimasa Uehara and Jun Ishikawa*
Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640, and 1Department of Molecular Function, Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan
(Received October 3, 2006. Accepted November 28, 2006)
*Corresponding author: Mailing address: Department of Bioactive Molecules, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan. Tel: +81-3-5285-1111, Fax: +81-3-5285-1272, E-mail: firstname.lastname@example.org
SUMMARY: We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.
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