Jpn. J. Infect. Dis., 60 (1), 45-47, 2007

To see a printable version of the article in the Adobe file format, click this [PDF] link.

Original Article

Construction of a Pair of Practical Nocardia-Escherichia coli Shuttle Vectors

Kazuhiro Chiba, Yasutaka Hoshino, Keiko Ishino, Takahisa Kogure1, Yuzuru Mikami1, Yoshimasa Uehara and Jun Ishikawa*

Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640, and 1Department of Molecular Function, Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan

(Received October 3, 2006. Accepted November 28, 2006)


*Corresponding author: Mailing address: Department of Bioactive Molecules, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan. Tel: +81-3-5285-1111, Fax: +81-3-5285-1272, E-mail: jun@nih.go.jp


SUMMARY: We constructed a pair of Nocardia-Escherichia coli shuttle vectors, pNV18 and pNV19, by combining the mycobacterial plasmid pAL5000 with the E. coli vector pK18 or pK19. These vectors have a number of useful features, including small size (4.4 kb), a multiple cloning site, and blue/white selection. To our knowledge, pNV18 and pNV19 are the first cloning vectors for practical use in Nocardia spp.


Go to JJID Homepage

Go to JJID 60 (1) Contents