Snapshot - 1APM

$C-/AMP$-DEPENDENT PROTEIN KINASE (E.C.2.7.1.37) ($C/APK$) (CATALYTIC SUBUNIT) "ALPHA" ISOENZYME MUTANT WITH SER 139 REPLACED BY ALA (/S139A$) COMPLEX WITH THE PEPTIDE INHIBITOR PKI(5-24) AND THE DETERGENT MEGA-8

Compound summary followed by details of each entity.

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      Resolution: 2.00 Å                        Deposition Date: 18-JAN-93

      Source: RECOMBINANT MOUSE (MUS $MUSCULUS) "ALPHA" 
              ISOENZYME S139A MUTANT EXPRESSED IN (ESCHERICHIA 
              $COLI) 
              
      Functional Class: TRANSFERASE(PHOSPHOTRANSFERASE)         

      Authors: D.R.KNIGHTON,S.M.BELL,J.ZHENG,L.F.TEN 
               *EYCK,N.-H.XUONG, S.S.TAYLOR,J.M.SOWADSKI 
               
      Primary Citation: D.R.KNIGHTON,S.M.BELL,J.ZHENG,L.F.TEN *EYCK, 
                        N.-H.XUONG,S.S.TAYLOR,J.M.SOWADSKI 2.0 ANGSTROM 
                        REFINED CRYSTAL STRUCTURE OF THE CATALYTIC SUBUNIT 
                        OF CAMP-DEPENDENT PROTEIN KINASE COMPLEXED WITH A 
                        PEPTIDE INHIBITOR AND DETERGENT TO BE PUBLISHED 
                        ASTM 353 
                        
      Number of Entities: 2

Entity 1APME

      Type: Protein                  Molecular Weight: 46710
      Number of Residues: 350
      Number of Alpha: 11            Content of Alpha: 35
      Number of Beta: 11             Content of Beta: 12

      Secondary structure by segment:

     LHLELELELELEHLHLELELHLHLELELELELHLHLHLHLHLHL

      Sequence and secondary structure:

   1 GNAAAAKKGS EQESVKEFLA KAKEDFLKKW ETPSQNTAQL DQFDRIKTLG 
              T HHHHHHHHHH HHHHHHHHHH HT      TG GGEEE EE E 

  51 TGSFGRVMLV KHKESGNHYA MKILDKQKVV KLKQIEHTLN EKRILQAVNF 
     E SSEEEEEE EETTTT EEE EEEEEHHHHH HTTTHHHHHH HHHHHHHT T 

 101 PFLVKLEFSF KDNSNLYMVM EYVAGGEMFS HLRRIGRFAE PHARFYAAQI 
     TTB  B EEE E SSEEEEEE E TGGGBHHH HHHHHT  TH HHHHHHHHHH 

 151 VLTFEYLHSL DLIYRDLKPE NLLIDQQGYI QVTDFGFAKR VKGRTWTLCG 
     HHHHHHHHHG TEE    TGG GEEETTTT E EE  TTTTEE  SS B    S 

 201 TPEYLAPEII LSKGYNKAVD WWALGVLIYE MAAGYPPFFA DQPIQIYEKI 
     THHHHHHHHH GTT BTGHHH HHHHHHHHHH HHHTS SS   STHHHHHHHH 

 251 VSGKVRFPSH FSSDLKDLLR NLLQVDLTKR FGNLKNGVND IKNHKWFATT 
     HHTT   TTT TTHHHHHHHH HHT STGGGG GGGGGGGHHH HHGGGGGGGT 

 301 DWIAIYQRKV EAPFIPKFKG PGDTSNFDDY EEEEIRVSIN EKCGKEFTEF 
     THHHHHHTTS   S      T GGGGGGT            SS   TTTGGGGT 



Entity 1APMI

      Type: Protein                  Molecular Weight: 2564
      Number of Residues: 20
      Number of Alpha: 1             Content of Alpha: 35
      Number of Beta: 0              Content of Beta: 0

      Secondary structure by segment:

     LHL

      Sequence and secondary structure:

   1 TTYADFIASG RTGRRNAIHD 
     THHHHHHHTS  SS