Snapshot - 1JSU

MOL_ID: 1; MOLECULE: CYCLIN-DEPENDENT KINASE-2; CHAIN: A; SYNONYM: CDK2; EC: 2.7.1.-; ENGINEERED: YES; MUTATION: PHOSPHORYLATED AT THR A 160; MOL_ID: 2; MOLECULE: CYCLIN A; CHAIN: B; FRAGMENT: RESIDUES 173 - 432; ENGINEERED: YES; MOL_ID: 3; MOLECULE: P27; CHAIN: C; FRAGMENT: RESIDUES 22 - 106; SYNONYM: KIP1, CIP2; ENGINEERED: YES

Compound summary followed by details of each entity.

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      Resolution: 2.30 Å                        Deposition Date: 03-JUL-96

      Source: MOL_ID: 1; ORGANISM_SCIENTIFIC: HOMO SAPIENS; 
              ORGANISM_COMMON: HUMAN; EXPRESSION_SYSTEM: 
              SPODOPTERA FRUGIPERDA INSECT CELLS; 
              EXPRESSION_SYSTEM_CELL_LINE: SF9; 
              EXPRESSION_SYSTEM_VECTOR: BACULOVIRUS; 
              OTHER_DETAILS: CYCLIN A-BOUND FORM PHOSPHORYLATED 
              ON THR 160 IN VITRO USING A CDK-ACTIVATING KINASE 
              CONSISTING OF THE CYCLINH-CDK7 COMPLEX; MOL_ID: 2; 
              ORGANISM_SCIENTIFIC: HOMO SAPIENS; 
              ORGANISM_COMMON: HUMAN; EXPRESSION_SYSTEM: 
              ESCHERICHIA COLI; EXPRESSION_SYSTEM_PLASMID: 
              PET3A; OTHER_DETAILS: THE FRAGMENT USED IN THE 
              CRYSTALLIZATION WAS PRODUCED BY THE CLEAVAGE OF 
              FULL-LENGTH CYCLIN A BY SUBTILISIN; MOL_ID: 3; 
              ORGANISM_SCIENTIFIC: HOMO SAPIENS; 
              ORGANISM_COMMON: HUMAN; EXPRESSION_SYSTEM: 
              ESCHERICHIA COLI; EXPRESSION_SYSTEM_PLASMID: PET3A 
              
      Functional Class: COMPLEX (TRANSFERASE/CYCLIN/INHIBITOR)  

      Authors: A.A.RUSSO,P.D.JEFFREY,N.P.PAVLETICH 
               
      Primary Citation: A.A.RUSSO,P.D.JEFFREY,A.K.PATTEN,J.MASSAGUE, 
                        N.P.PAVLETICH CRYSTAL STRUCTURE OF THE P27KIP1 
                        CYCLIN-DEPENDENT-KINASE INHIBITOR BOUND TO THE 
                        CYCLIN A-CDK2 COMPLEX NATURE V. 382 325 1996 ASTM 
                        NATUAS UK ISSN 0028-0836 0006 
                        
      Number of Entities: 3

Entity 1JSU:A

      Type: Protein                  Molecular Weight: 39160
      Number of Residues: 298
      Number of Alpha: 9             Content of Alpha: 30.20
      Number of Beta: 9              Content of Beta: 11.41

      Secondary structure by segment:

     LELELHLELELEHLHLELELELELHLHLHLHLHLHL

      Sequence and secondary structure:

   1 MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR 
                    S    E EE SSSS EE EEEEEE BTT TTBSTHHHHH 

  51 EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP 
     HHHHHGGT T TTB  B EEE E SSEEEEEE E  SEEHHHH HHHHHHHT T 

 101 LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINTEGA IKLADFGLAR 
     HHHHHHHHHH HHHHHHHHHG GTEE    TG GGEEETTTT  EEE  TTTTE 

 151 AFGVPVRTY* HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR 
     E  SS      SS STGGGTT HHHHTTTS   TGHHHHHHHH HHHHHHHHTS 

 201 ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF PKWARQDFSK 
     TTTT STHHH HHHHHHHHHT   TTTTTGGG GGGGGGGGGT       THHH 

 251 VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRL 
     HGGGTTHHHH HHHHHHTTST TTTT THHHH GGGGGGGGT      SS   



Entity 1JSU:B

      Type: Protein                  Molecular Weight: 34498
      Number of Residues: 260
      Number of Alpha: 13            Content of Alpha: 67.31
      Number of Beta: 0              Content of Beta: 0.00

      Secondary structure by segment:

     LHLHLHLHLHLHLHLHLHLHLHLHLHL

      Sequence and secondary structure:

   1 NEVPDYHEDI HTYLREMEVK CKPKVGYMKK QPDITNSMRA ILVDWLVEVG 
       TGGGHHHH HHHHHHHHHH T  TGGHHHH TSS THHHHH HHHHHHHHHH 

  51 EEYKLQNETL HLAVNYIDRF LSSMSVLRGK LQLVGTAAML LASKFEEIYP 
     HHTTTTHHHH HHHHHHHHHH HHHT  THHH HHHHHHHHHH HHHHHHTSS  

 101 PEVAEFVYIT DDTYTKKQVL RMEHLVLKVL TFDLAAPTVN QFLTQYFLHQ 
      THHHHHHHH HT STHHHHH HHHHHHHHHT TTT    THH HHHHHHHGGT 

 151 QPANCKVESL AMFLGELSLI DADPYLKYLP SVIAGAAFHL ALYTVTGQSW 
     SS THHHHHH HHHHHHHHHH HHHHHGGGGH HHHHHHHHHH HHHHHHT    

 201 PESLIRKTGY TLESLKPCLM DLHQTYLKAP QHAQQSIREK YKNSKYHGVS 
     THHHHHHHT  THHHHHHHHH HHHHHHHHHH HTS THHHHH HHGGGGGGGG 

 251 LLNPPETLNL 
     GT   SS    



Entity 1JSU:C

      Type: Protein                  Molecular Weight: 11447
      Number of Residues: 84
      Number of Alpha: 2             Content of Alpha: 21.43
      Number of Beta: 2              Content of Beta: 4.76

      Secondary structure by segment:

     LHLHLELEL

      Sequence and secondary structure:

   1 HPKPSACRNL FGPVDHEELT RDLEKHCRDM EEASQRKWNF DFQNHKPLEG 
        TTTT  S S   THHHHH HHHHHHHGGG GHHHHHHIIE EIIIIEE SS 

  51 KYEWQEVEKG SLPEFYYRPP RPPKGACKVP AQES 
     SS     TTT TTTGGGGT