MOLECULE: PROTEIN KINASE C DELTA TYPE; DOMAIN: CYS2;
EC: 2.7.1.-; HETEROGEN: ZINC; HETEROGEN:
Protein kinase Cs (PKCs) are a ubiquitous family of regulatory enzymes that associate with
membranes and are activated by diacylglycerol or tumor-promoting agonists such as phorbol
esters. The structure of the second activator-binding domain of PKC delta has been determined in
complex with phorbol 13-acetate, which binds in a groove between two pulled-apart beta strands
at the tip of the domain. The C3, C4, and C20 phorbol oxygens form hydrogen bonds with
main-chain groups whose orientation is controlled by a set of highly conserved residues. Phorbol
binding caps the groove and forms a contiguous hydrophobic surface covering one-third of the
domain, explaining how the activator promotes insertion of PKC into membranes.
 Zhang G; Kazanietz MG; Blumberg PM; Hurley JH (1995) Crystal structure of the cys2 activator-binding domain of protein kinase C delta in complex with phorbol ester.
Cell 81: 917. [Abstract]
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