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In gel kinase assay



I wonder if anyone has any experience doing in-gel kinase assays for 
PKC?  Specifically we are interested in PKC alpha, delta and zeta.   
We have tried them with little success and would appreciate any 
advice.  Thanks.  Mary Reyland
Mary E. Reyland, Ph.D.
Assistant Professor
Department of Basic Sciences and Oral Research, Box C286
University of Colorado HSC
4200 E. Ninth Ave
Denver, CO  80262
Phone:(303) 315-3236
Fax: (303) 315-3013
mary.reyland@uchsc.edu