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Re: Calcium

I have also been studying MBP phosphorylation with a 38 kDa MAPKinase from
the moss Funaria hygrometrica and have made the same observation. I have
avoided adding CA2+ since it is known that MAPkinases are
Ca2+-independent. There is one report in the plant kingdom which shows the
reqiurement for Ca2+ -- for a MAPkinase that is. I too am not sure about
this whole issue. But my results indicate that the MAPkinase I am dealing
with shows the same trend with or without Ca2+. So, that's the story. I
personally feel you can avoid Ca2+.
Jacinta D'Souza-Saldanha,
Dept. of Biological Sciences,
Tata Institute of Fundamental Research,
Homi Bhabha Road,
Colaba, Mumbai-400 005,

On Fri, 22 Jan 1999, Yves wrote:

> Dear colleagues,
> Testing the autophosphorylation capacity and the ability to phosphorylate
> MBP from our plant MAP3Kepsilon (still unpublished), we used Mg2+ : it
> works and Ca2+ : it works too, but shows a quite different result. As a
> general rule, people use mostly Mg2+ (or Mn2+). Is there any reason to
> avoid Ca2+ ?
> Yves
> Dr Yves HENRY
> Laboratoire BDP, UMR CNRS 8618,
> IBP, Bātiment 630, UPS
> 91405 Orsay cedex
> tel : 01 69 33 63 93
> mail : yhenry@ibp.u-psud.fr