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How clean is your enzyme? Calcium might be affecting association with
something in your sample, or calcium might be activating another kinase in
What do you mean you get a difference?
The paradigm is that you need Mg or Mn to coordinate the phosphates on the
ATP in the correct manner for binding to the kinase and subsequent
hydrolysis. If you have an enzyme that doesn't require this (and I
wouldn't expect a kinase similar to the eukaryotic kinases to be in this
class), then its conceivable that the calcium might coordinate the
phosphates, but you'd have to do a bit of work to prove this.