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how to know the members of same family


I Would like to ask that whether it is possible to know that how many 
members are present for a particular family of kinase(s) by using 
biochemical techniques.

Whether mol. weight of kinase  could be lower (10-20 KD) than the have  
predicated of the protein (from sequence.

I will highly appreciate to recevie any information on these lines.

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