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Re: posphoprotein mobility in SDS gels


   I think this has been discussed previously on this forum. It turns out
that you have cases where the phosphorylated version of a protein
migrates faster than the unphosphorylated form and vice versa. Sometimes
they also comigrate. One explanation for the slower mobility of the
phosphorylated form is that due to charge repulsion less SDS can bind to
the protein. Then there is always the possibility of a difference in
folding that can also affect the mobility in SDS gels.

Chris (turck@itsa.ucsf.edu)