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Overlaying gels

Re: Overlaying gels

In my lab we use isopropanol instead of water-satd-isobutanol to overlay
gels.  We switched to this from isobutanol a couple of years ago since it
was not necessary to saturate with water and therefore easier to use. Also
it smells better than isobut.  It works fine.  Besides, your're less likely
to get confusion over how much water to add to the isobutanol/ how much to
shake it up etc.etc.