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Re: In Gel KAs




Most protein kinases prefer ATP above GTP. You can easily find out by 
letting GTP compete for the 32-P-labeled ATP in your reaction mix. With 
the protein kinase I'm working on (MARK) we need quite high GTP 
concentrations to see an effect.

Comercially available PKA catalytic subunit is a suitable control for 
In-Gel-KAs. Supplied for example by Promega.

Good luck!

Gerard


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Dr.Gerard Drewes                          Phone: (+49)(0)40-89984766 office
Max-Planck-Society                               (+49)(0)40-8515181  home
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