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detection



Hello all,

can anybody tell me, if there is another detection possibility of 
kinase activity except radiolabelled ATP?
I think about antiphosphoSer/Thr antibodies, but don't  know 
whether the immunodetection is sensitive and specific enough if we 
use purified enzyme and add protein as a substrate to 1mg/ml.
Thanks,




Vladimir Krystof
---------------------------------------------------
Laboratory of Growth Regulators
Palacky University
Slechtitelu 11
CZ-78371 Olomouc
Czech Republic

phone: ++420 68 5222487
fax:   ++420 68 5221357

http://prfholnt.upol.cz:81/growthreg