[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]
Turnover number for your favorite kinase
I am trying to get an idea of how 'fast' and how close to saturation
various kinases are. This is for a course I teach on Cell Signaling.
Could you help me with the following info for your favorite kinase?
Concentration of Yfk in some cell type
Identity and concentration of a physiological substrate
Vmax value (preferably using a physiological substrate)
Here's one to start:
MEK1/MKK1 is ~ 1 uM in many vertebrate cell types (1)
Its substrates ERK1/ERK2 are also about ~ 1 uM (1)
Vmax has been estimated to be 1 to 10 min-1 for wt MKK1 activated
by Mos or Raf-1 (2-4)
Km ~ 0.34 uM with ERK2 as substrate (2-4)
Vmax/Km is ~ 3-30 min-1 uM-1 (2-4)
(1) Ferrell (1996) TiBS 21:460-466.
(2) Mansour et al. (1996) Biochemistry 35:15529-15536.
(3) Alessi et al. (1994) EMBO J 13:1610-1619.
(4) Dent et al. (1994) Biochem J 303:105-1122.
James E. Ferrell, Jr., M.D., Ph.D.
Department of Molecular Pharmacology
Stanford University School of Medicine
Stanford CA 94305-5332
Phone: 650 725-0765 [PLEASE NOTE NEW AREA CODE]
Fax: 650 725-2952