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Turnover number for your favorite kinase



Dear listmates,

I am trying to get an idea of how 'fast' and how close to saturation
various kinases are.  This is for a course I teach on Cell Signaling.
Could you help me with the following info for your favorite kinase?

	Concentration of Yfk in some cell type
	Identity and concentration of a physiological substrate
	Vmax value (preferably using a physiological substrate)
	Km (ditto)

Here's one to start:

	MEK1/MKK1 is ~ 1 uM in many vertebrate cell types (1)
	Its substrates ERK1/ERK2 are also about ~ 1 uM (1)
	Vmax has been estimated to be 1 to 10 min-1 for wt MKK1 activated
by Mos or Raf-1 (2-4)
	Km ~ 0.34 uM with ERK2 as substrate (2-4)
	Vmax/Km  is ~ 3-30 min-1 uM-1 (2-4)

Refs:
(1) Ferrell (1996) TiBS 21:460-466.
(2) Mansour et al. (1996) Biochemistry 35:15529-15536.
(3) Alessi et al. (1994) EMBO J 13:1610-1619.
(4) Dent et al. (1994) Biochem J 303:105-1122.

Cheers,

Jim


-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
James E. Ferrell, Jr., M.D., Ph.D.
Assistant Professor
Department of Molecular Pharmacology
Stanford University School of Medicine
Stanford CA  94305-5332
ferrell@cmgm.stanford.edu
Phone:  650 725-0765 [PLEASE NOTE NEW AREA CODE]
Fax:  650 725-2952