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phosphatase treating proteins in vitro



Hi,

Does anyone have a good protocol for phosphatase treating proteins (to
dephosphorylate ser / thr residues) with lambda phosphatase or CIAP (or
otherwise).

Thanks,

Michael...
______________________________________________________________________________

Dr Michael-Christopher Keogh,
Bldg C1, Rm 207,
Dept of Biological Chemistry and Molecular Pharmacology,
Harvard Medical School,
240 Longwood Ave,
Cambridge, MA 02115,
USA.
Tel.       	617 432 0366
Fax.       	617 738 0516
Email.  	Michael_Keogh@hms.harvard.edu
______________________________________________________________________________