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Re: CDK kinetics




Indeed, excessive amounts of ATP do inhibit many protein kinases (usually 
in the range from 4 mM upwards). We once characterized ERK2 and found 
max turnover around 1-2 mM, declining again sharply over 3 mM.
A (trivial) explanation might be sort of a "traffic jam" at the ATP 
binding site. 
Also, one must make sure to include always an excess of Mg++ over ATP, 
since free ATP is not a substrate.

Hope this helps,


Gerard

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Dr.Gerard Drewes                          Phone: (+49)(0)40-89984766 office
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On Thu, 22 Apr 1999, Vladimir Krystof wrote:


> Is it possible that excessive amount of ATP inhibits CDK2 activity? 
> Or maybe the result are not interpreted in the right way?
> Thanks in advance.
> 
> 
> 
> 
> Vladimir Krystof
> ---------------------------------------------------
> Laboratory of Growth Regulators
> Palacky University
> Slechtitelu 11
> CZ-78371 Olomouc
> Czech Republic
> 
> phone: ++420 68 5222487
> fax:   ++420 68 5221357
> 
> http://prfholnt.upol.cz:81/
>