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PKC inhibitors

Dear Kinase Folk,

We are using a variety of PKC inhibitors to inhibit PKC isoforms in whole
mouse embryo culture.  We are keen to confirm which isoforms are inhibited
whilst using  the concentrations recommended by the manufacturers.  Can
anyone tell me whether PKC isoforms inhibited in culture would remain
turned off in an in vitro assay after immunoprecipitation?  If not would it
be valid to add an extract of the culture medium to the assay?



Andrew, M. Hynes PhD,
Neural Development Unit,
Institute of Child Health,
30 Guilford Street,
London WC1N 1EH,
United Kingdom
Tel: 44-171-905-2230
E-mail: A.Hynes@ich.ucl.ac.uk