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Re: Gamma-ATP

Sigma sells a ready-to-use gamma-phosphate-linked ATP affinity sepharose.
I would be very interested if this works. In our hands, early batches from
UBI worked out fine, while later batches didnt, and other researchers have
experienced the same.


Dr.Gerard Drewes                          Phone: (+49)(0)40-89984766 office
Max-Planck-Society                               (+49)(0)40-8515181  home
Unit for Structural Molecular Biology     Fax:   (+49)(0)40-89716810
c/o DESY, Hasylab 25B                     e-mail drewes@mpasmb.desy.de
Notkestrasse 85
D-22603 Hamburg

On Fri, 21 May 1999 ksrivenu@notes.mdacc.tmc.edu wrote:

> Dear Kinase Researchers:
> I am looking for a commercial source to purchase
> adenosine-5'(gamma-4-aminophenyl)-triphosphate to prepare a affinity column
> for protein kinases.  Literature indicates that  gamma-phosphate linked ATP
> is structurally  a sound  ligand for specific binding of protein kinases
> (EJB 214, 459-467, 1993, and others). I do know  that USB was marketing it
> until 2 years ago, but have discontinued. If anyone knows a company that
> has gamma-aminophenyl ATP either in the US or abroad, please let me know.
> Thanks for your help.
> K.S. Srivenugopal, Ph.D.
> MD Anderson Cancer Center
> Houston, TX
> email: ksrivenu@mdacc.tmc.edu