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PhosphoSpot



Dear kinasers,

has anyone experiences with the use of "PhosphoSpot"-filters (from
Jerini) to identify kinase activity in cellular extracts???
If so, is it possible to detect phosphorylated kinase substrates on this
filters with antibodies against PTyr, PSer, or PThr? Do you know a good
working protocol?

Thanks a lot.

Best regards,
Brigitte