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PKC inhibitors



We are looking at the effect of Ro-318220 and other inhibitors on TPA
activation of PKC isomers in cultured cells.  We assess PKC activation
by measuring PKC translocation form cytosol to membrane on Western
blots.  We find that Ro-318220 (and some other inhibitors) alone cause a
redistribution and apparent activation in the absence of TPA.  Any
comments on using this methodology to assess inhibitor specificity are
welcome.

Seamus A Rooney