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MAP kinase protocol
Is there anybody who did MAP kinase assay with P-32-ATP, MBP, filter binding?
What buffer should I use for best results?
I used 50mM Hepes-HCl pH8.0, 2.0 mMDTT, 0.1 EGTA, 5mM Mg(Ch3COO) buffer but
it seems to me that the binding system does not work.
Can anybode give me a good hint why it could happen?
Thanks in advance,
Alex Lyakhovich, M.D.
University of Tampere