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Dear Kinasers

we are using the Dual luciferase system taking advantage of the
"promoterless" Renilla luciferase gene to monitor transfection efficiency
in a ras/kinases/growth "independent" way. The protocol requires quenching
of the Renilla luciferase before measuring of the firefly luciferase (your
test gene). There is a company that sells the quenching/restarting
solution, but the kit is amazingly expensive. Does anybody know what one
can use instead (or what is in the kit solution)?
Please let me know!

Giulio

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Giulio Superti-Furga, PhD
Developmental Biology Programme
European Molecular Biology Laboratory
Meyerhofstrasse 1
690117 Heidelberg, Germany
+49 6221 387 357 phone
+49 6221 387 166 fax
superti@embl-heidelberg.de