[Date Prev][Date Next][Thread Prev][Thread Next][Date Index][Thread Index]

No Subject

Dear Kinasers

we are using the Dual luciferase system taking advantage of the
"promoterless" Renilla luciferase gene to monitor transfection efficiency
in a ras/kinases/growth "independent" way. The protocol requires quenching
of the Renilla luciferase before measuring of the firefly luciferase (your
test gene). There is a company that sells the quenching/restarting
solution, but the kit is amazingly expensive. Does anybody know what one
can use instead (or what is in the kit solution)?
Please let me know!



Giulio Superti-Furga, PhD
Developmental Biology Programme
European Molecular Biology Laboratory
Meyerhofstrasse 1
690117 Heidelberg, Germany
+49 6221 387 357 phone
+49 6221 387 166 fax