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kinases Immunoprecipitation kinase assay for PKA?



I have been measuring PKA activity in cells after sonication in a buffer
that contains no detergent. After sonication, the lysates are cleared by
centrifugation, leaving a substantial pellet of insoluble material. The
supernatant is then assayed for PKA activity (four reactions: -/+ Kemptide,
-/+ PKI).

Has anybody ever successfully assayed PKA from cells lysed in a
detergent-containing buffer (e.g. NP40 or Triton X100 at 0.5 to 1%)? Is
there a way to IP PKA and then assess its activity, like is done for myriad
other kinases (e.g. ERKs, CDKs, RSKs,...)?

Any insights are greatly appreciated.

Alan 

---------------
Alan K. Howe, Ph.D.
University of North Carolina at Chapel Hill
Lineberger Comprehensive Cancer Center
Dept. of Pharmacology, CB# 7365
Mary Ellen Jones Bldg, Rm 920
Chapel Hill, NC   27599-7365
(919) 966-4343
(919) 966-5640 (FAX)
alanhowe@med.unc.edu