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Does anybody have a solution for reducing the background on a phosphotyrosine
We have i.p. with an anti-VEGF-R antibody (Santa Cruz) and blot with monoclonal
4G10 (UBI) phosphotyrosine antibody. We have also tried with PY-20
We have tried to block in 5% skimmilk or 3% BSA. We do the washing with PBS+0.1%
Tween-20, and develop with ECL.
Also, is there anybody doing a VEGF-R kinase assay using phosphotyrosine
antibodies or phosphorylation of a substrate such as Poly-Glu-Tyr?
Marianne Scheel Fjording
LEO Pharmaceutical Products