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kinases Acid-denaturing enolase

Could anyone out there provide the/a protocol for acid-denaturing enolase
for Src kinase assays?

Thanks, in advance, for your help.

P.S. Thanks to everyone that responded to my earlier PKA IP kinase assay
question. I think I've got it working!
Alan K. Howe, Ph.D.
University of North Carolina at Chapel Hill
Lineberger Comprehensive Cancer Center
Dept. of Pharmacology, CB# 7365
Mary Ellen Jones Bldg, Rm 920
Chapel Hill, NC   27599-7365
(919) 966-4343
(919) 966-5640 (FAX)