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Re: kinases In gel kinase



>Hi,
>
>I came across a 116 KD band when i performed ingel assay. Does anybody
>know the list of protein kinases of about 116kD ? If not, does anybody
>know how to get the list of kinases of 116 kD
>
>
>thanks
>
>vidya Mamidipudi
>Graduate student
>Department of Biological Sciences
>Auburn university, Al

Dear Vidya

High on my list of suspects would be the Rsk proteins (Rsk1, Rsk2, Rsk3),
which migrate at around 90 kDa when active and do show up in in-gel kinase
assays.  Santa Cruz sells decent Rsk antisera.

Cheers

Jim Ferrell

-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
James E. Ferrell, Jr., M.D., Ph.D.
Associate Professor
Department of Molecular Pharmacology
Stanford University School of Medicine
Stanford CA  94305-5332
ferrell@cmgm.stanford.edu
Phone:  650 725-0765
Fax:  650 725-2952